Multi-chamber IV bag has two categories: fluid multi-chamber IV bag and fluid-powder multi-chamber IV bag. It’s defined as the separate storage of different medicine in different sub-chambers by welding during production, storage and transportation. In application, the separate sub-chambers are connected by external forces, and the contents are mixed. Multi-chamber IV bag is mainly used for the contents that are unstable after mixing, and can not guarantee long term storage. This article analyzes the testing of two key factors for multi-chamber IV bags: welding and barrier property.